Plasmid_Backbone

Part:BBa_K2259081:Design

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-02)


Base vector 2.0 - a backbone for SynORI multi-plasmid framework


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 968
    Illegal suffix found at 2107
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 968
    Illegal NheI site found at 745
    Illegal NheI site found at 2283
    Illegal SpeI site found at 2108
    Illegal PstI site found at 2122
    Illegal NotI site found at 974
    Illegal NotI site found at 2115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 968
    Illegal BglII site found at 1451
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 968
    Illegal suffix found at 2108
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 968
    Plasmid lacks a suffix.
    Illegal XbaI site found at 983
    Illegal SpeI site found at 2108
    Illegal PstI site found at 2122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Because original base vector is no longer available due to ccdB toxin it contained, we have changed ccdB toxin selection into a blue/white selection


Source

Based on base vector BBa_I51020.

References